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BL21 E. coli are by far the most obnoxious bacteria genetic engineers usually work with for 1 simple reason. They are maddeningly difficult to modify and load with new DNA. This wouldn't be an issue if they weren't also one of the best types for producing proteins en mass, and as such have application in everything from pharmaceutical production, to producing ingredients for foods. But after a few years of tinkering with it I finally found a protocol that reliably fixes this irritating issue, and makes it easy to transform them reliably.

Written protocol - https://github.com/thethoughtemporium...

Sebastian's spinner - https://www.tinkercad.com/things/5t5G...

Previous videos;
Modifying Bacteria -    • Simple Bacteria Genetic Engineering  
Modifying yeast -    • Making Bread Healthier Using Genetic ...  
Making a heat block/incubator -    • From Beer Cooler to a DIY Incubator  
Growing spider silk -   • I Grew Real Spider Silk Using Yeast  
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