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Genetic Engineering is, amazing. In many previous videos we've explored the basics of how genetic engineering works, and today we'll be exploring something a little more complicated; engineering yeast to produce beta-carotene, the precursor for vitamin A. We'll then use these newly modified yeast to bake and brew with, giving the items we make a vitamin boost!

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Igem Team: http://2011.igem.org/Team:Johns_Hopkins
Original Paper: https://www.ncbi.nlm.nih.gov/pmc/arti...

Merch: https://teespring.com/stores/the-thou...

PLATE solution recipe:
40% PEG3350 (w/v)
100 mM lithium acetate (LiAc)
10 mM Tris, pH 7.5
0.4 mM EDTA
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Previous videos:
Genetically modify Bacteria    • Simple Bacteria Genetic Engineering  
Building an Incubator    • From Beer Cooler to a DIY Incubator  
Spider Beer 1    • Spider Beer - Making Yeast Produce Sp...  
DNA Extraction    • Extracting Spider/Bacteria DNA Using ...  
Growth Media    • Growth Media and Pouring Plates - Mic...  
PCR    • Everything You Could Want To Know Abo...  
Fluorescent Microscope    • Fluorescent microscopes are amazing!  
Gel electrophoresis    • Gel Electrophoresis: How It Works and...  
How DNA was Discovered    • The Experiment That Discovered DNA By...  
How to stock a genetics lab    • How to Stock a Biology/Genetics Lab  
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