Join Bruce Bryan as he takes part in a nationwide monitoring program testing for antibiotic resistance genes in environmental soil samples. We’ll demonstrate how you can select a sampling strategy based on your own hypotheses about antibiotic resistance hotspots, go to the field and sample, extract total environmental DNA from soil, and use the molecular methods of PCR and gel electrophoresis to test samples for evidence of tetracycline resistance.

Webinar review questions: https://docs.google.com/document/d/1O...

PARE Project: https://sites.tufts.edu/ctse/pare/

miniPCR® Antibiotic Resistance Lab: Monitoring resistant organisms in the environment (previous case study based lab): https://www.minipcr.com/product/antib...

Webinar slides: https://minipcr.com/wp-content/upload... DNA Analysis Project_web.pptx

Free PCR and gel electrophoresis teaching resources: https://www.minipcr.com/tutorials​

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Questions? Contact [email protected]