Mouse brain cleared with CUBIC

Piece of human cortex (occipital lobe V2 of visual cortex) from 90 y.o. male donor

One hemisphere of a mouse brain with sparse neuronal labeling (Thy1)

Glutamatergic neurons in the mouse brain (sparse labeling)

Mouse brain stained for amyloid plaques (green) and arteries (magenta).

Contrast map of Olympus XLFluor 4x/0.28

Light-sheet excitation arm components

Mouse brain with spinal cord imaged at 5x magnification.

Whole mouse imaged at 0.9x magnification

Chicken embryo imaged at 1.2x magnification

Nervous system of Xenopus tadpole imaged at 5x magnification.

Benchtop mesoSPIM detection arm assembly (exploded view)

Mounting Thorlabs galvo mirror and heatsink for Benchtop mesoSPIM excitation arm.

Benchtop mesoSPIM assembly.

Mollusc-inspired multi-immersion microscope objectives

Johannes Paetzold: Machine learning for vascular structures in microscopic data.

Stephan Preibisch: Towards reconstruction of peta-scale lightsheet microscopy datasets.

Benjamin Schmid: Intuitive rendering of 3D animations from microscope data using 3Dscript.

Laura Batti: ALICe, Advanced Lightsheet Imaging Center.

José María Mateos: Experiences running the MesoSPIM in a Core Facility.

Thomas Naert: mesoSPIM and U-Net deep learning: three-dimensional modeling of embryonic development

Nikita Vladimirov: mesoSPIM development and updates, testing the lens field curvature.

Nikita Vladimirov: Demo of mesoSPIM software and image acquisition workflow

Sven Hildebrand: Investigating the human neocortex in 3D...

Shan Zhao: The challenges of human tissue clearing, imaging and application.

Etsuo Susaki: CUBIC for whole-organ/body cell circuit and tissue architecture analysis.

Alba Vieites Prado: iDISCO+ tissue clearing and ClearMap for the analysis of axonal projections...

Fritjof Helmchen "Welcome" talk at the 1-st mesoSPIM User Meeting, March 10, 2022.

Stitching 3D images from tiles using Fiji/BigStitcher

ETL adjustment trick using the laser beam