Despicable Me: Minion Mayhem is a simulator ride attraction at Universal Studios Florida and Universal Studios Hollywood. The attraction is based on Universal Studios and Illumination Entertainment's 2010 animated film Despicable Me and employs the use of 3-D HD digital animation. The attraction replaced Jimmy Neutron's Nicktoon Blast in Florida and Terminator 2 3D in Hollywood.
Universal Orlando Resort announced that the attraction is being designed by their Universal Creative company in association with Illumination Entertainment and Despicable Me film producer Chris Meledandri. The ride is a motion simulator and employs the use of high-definition 3-D Infitec projection systems, and features multiple characters from the film, including Gru, Margo, Edith, Agnes and the Minions.

The attraction at Universal Studios Florida is located in Soundstage 42 in the Production Central area. The soundstage previously housed The Funtastic World of Hanna-Barbera 1990 until 2002 and Jimmy Neutron's Nicktoon Blast from 2003 until 2011. The attraction at Universal Studios Hollywood is located on the former site of T2 3-D: Battle Across Time on the Upper Lot.

After the ride, the door opens to a dance party with the public dancing to "Boogie Fever," along with a costumed Minion.
Voice cast
Steve Carell as Gru
Miranda Cosgrove as Margo
Dana Gaier as Edith
Elsie Fisher as Agnes
Pierre Coffin as the Minions.
Dr. Nefario appears as a non-speaking cameo.