How Can You Prevent Hop Latent Viroid HLVd From Spreading?
Professor DeBacco

Additional Video Sources:
Medicinal Genomics. (2023a, June 8). Understanding and Managing HOP latent viroid in cannabis - Zamir Punja, PhD [Video]. YouTube.    • Understanding and Managing Hop Latent...  

Starts with Testing/Screening
Knowing what you have
How Can You Prevent HLVd from Spreading?
Strict and consistent tool sterilization
PPE for workers
Rigorous testing protocol
Repeat testing of Mother room

Plant Eradication
Removing plants that test positive and removing them, followed by continual testing can reduce the presence of HLVd in a growing operation.
Cleaning Tools and Surfaces
This should be done on a regular basis.
However the product you select as your “cleaner” may make the area look clean but many not have any impact on HLVd

Not Effective Methods
Alcohol
Alcohol + flame
Flaming tools
H2O2
Acetic Acid (Vinegar)
Ammonium cleaners (Ex. Lysol)
UV Light
UV-C exposure on leaves and roots for 5min. And viroid was still present

What Does Work… (On Tools and Surfaces)
Virkon S 2%
Household Bleach 10-20% for 30-60sec.
Common lab saying… 10% bleach for 10min.
Mix and use the same day.

Same Can Not Be Applied to Root (Plant) Tissue
Previous products and recommendations are for tools and surfaces and those effective in those situations are not effective at cleaning plant tissue.
UV-C Irradiation
The viroid is very stable in plant tissue.
This makes it very hard to “clean” plant material which is why it should be bagged and physically removed.
Sap is much more Difficult
The “best” method for sap containing viroids may be Nucleases

Link to Lecture Slides: https://drive.google.com/file/d/1bKoK...

*Due to the description character limit the full work cited for "How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?" can be viewed at... https://docs.google.com/document/d/1v...