Tags: Tissue Culture, Propagation, Cultivation

Mark Jordan presents "Cannabis Tissue Culture and Germplasm Storage" at CannMed 2019.
After starting off in 1902, Cannabis tissue culture is quickly becoming a mainstream form of commercial Cannabis propagation. There are several hurdles that propagators need to overcome in order to successfully perform tissue culture on Cannabis. Once in culture, the possibilities are endless. Addressing these issues and understanding the applications that tissue culture presents are key to the advancement of the industry as a whole. Mark has developed protocols over the years and plans to shed some light on the problems and solutions of the cannabis tissue culture industry, along with addressing some of the applications such as germplasm storage along with data surrounding the costs and benefits.

0:00 Introduction
0:50 Why use tissue culture on Cannabis?
3:25 How do you know what chemical concentrations to add?
4:43 Understanding plant morphology
5:39 The 4 Common Stages of Plant Tissue Culture
6:53 Initiation
7:37 Meristem Culture For Virus Elimination
8:36 Stage 1 - Axillary Node Culture
9:47 Multiplication
11:55 Future Shipping
14:22 Acclimation - Herdening
15:23 The 4 main problems with Cannabis TC
16:24 1.2 Better Cytokinin Auxin combinations
17:01 Endophytes
17:53 2.1 More Pathogen Pictures
18:10 2.2 Pathogen Elimination Explant Prep
18:48 Common Stage 3 - Rooting Problems
20:56 Common Stage 4 - Acclimation Problems
21:39 Germplasm Conservation
21:57 Germplasm Storage - Refrigeration and Freezing of Cultures
23:34 Plant Breeding