A more responsible use of antibiotics is an important approach required to prevent multi-resistant pathogens. For that one needs to identify the pathogen and also detect the respective antibiotic resistance. If we know about the vulnerability of the specific bacterium, a more targeted treatment is possible. In this video our guest speaker Esther (from iGEM Hamburg) presents three different methods to detect antimicrobial resistance.

1. Disc Diffusion method
2. E-Test
3. Colorimetric tests



References:

Interview with Prof. Dr. Axel Hamprecht (stellvertretender Vorsitzender des Nationalen Antibiotika-Sensititvitätstest-Komitees (NAK))

Khan, Z.A., Siddiqui, M.F., Park, S., 2019. Current and Emerging Methods of Antibiotic Susceptibility Testing. Diagnostics (Basel) 9, 49. https://doi.org/10.3390/diagnostics90...

Yalew, S.T., 2020. Review on Antibiotic Resistance: Resistance Mechanisms, Methods of Detection and Its Controlling Strategies. BJSTR 24. https://doi.org/10.26717/BJSTR.2020.2...

Kowalska-Krochmal, B., Dudek-Wicher, R., 2021. The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens 10, 165. https://doi.org/10.3390/pathogens1002...

https://www.who.int/news-room/fact-sh...

F.C. Tenover,Antimicrobial Susceptibility Testing☆,Editor(s): Thomas M. Schmidt,
Encyclopedia of Microbiology (Fourth Edition),Academic Press,2019,Pages 166-175,
ISBN 9780128117378,https://doi.org/10.1016/B978-0-12-801....



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Music:
Corbyn Kites - Blurry Vision
   • Video  



Thanks for the great support and research: Esther Lange
iGEM Hamburg:   / igem_hamburg  



Time Codes:
0:00 Introduction
0:29 Antibiotic Resistance
1:19 Why to prescribe the correct antibiotic
1:55 Disc Diffusion method
2:57 E-Test
4:32 Colorimetric tests



#Antibiotics #Resistance #Detection #antibioticresistance